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Cell Culture Products
Low Cell Adhesion Products PrimeSurface™

Products

Made in Japan

The inner wall of PrimeSurface products are coated with our unique ultra hydrophilic polymer, which prevents a cell from adhering to the plastic surface induces a spontaneous cell spheroid formation in equal size and shape.

Features

  • Ideal tool for uniform spheroid cell culture formation
  • The uniform spheroid cell culture could be effective formed due to the adhesion of cell is inhibited by our unique surface coating technique of PrimeSurface product.
  • Ideal tool for differentiation study
  • Inducer reagent for differentiation can be applied after EB body formation of ES cells.
  • The chemical bonding of ultra-hydrophilic coating polymer on our product is stable culture surface which makes no elution of chemicals from substrate.
  • Ideal for differentiation and stimulation response studies

Applications

Medical Research Institution/Laboratory

Cell Culture

For Stem Cell Research:

Induction of ES, iPS and mesenchymal stem cells differentiation by embryoid body formation.

For Drug Screening Research & Development:

An ideal product for drug response studies. Three dimension spheroid models are more physiologically relevant than two dimension monolayer model.

Specifications

Cat. NoProductDescriptionCulture AreaVolumeQty/PkQty/Cs
MS-90350 PrimeSurface Dish 3535 Φ × 14(H)mm9 cm2- 5 50
MS-90600 PrimeSurface Dish 6060 Φ × 15(H)mm21 cm2- 10 120
MS-90900 PrimeSurface Dish 9090 Φ × 20(H)mm57 cm2- 10 50
MS-90240 PrimeSurface Plate 24F24wells, Flat1.8 cm23.4mL/well 1 10

Remark

  • Storage: Room temperature.
  • Expiration: 2 years after production
Cat. NoProductWellsBottomVolumeQty/PkQty/Cs
MS-9384U PrimeSurface 384U384U bottom0.1mL1 20
MS-9384W PrimeSurface 384U White Plate384U bottom0.1mL 1 20
MS-9096VZ PrimeSurface 96V96V bottom0.3mL1 20
MS-9096MZ PrimeSurface 96M96Spindle bottom0.2mL 1 20
MS-9096UZ PrimeSurface 96U96U bottom0.3mL1 20
MS-9096WZ PrimeSurface 96U White Plate 96U bottom0.3mL 1 20

Remark

  • Storage: Room temperature.
  • Expiration: 2 years after production

Experiments

3D spheroid culture using HepG2 cell line
-comparison data-

Cell line :   HepG2
Seeding density : 1000 cells/well
Medium : DMEM+10%FBS
Culture period : 3 days
Plate : 96 well U plates for 3D culturing from different companies

Number of Spheroids

Classification based on microscopic images

[Grade 1]

Single spheroid from single well

[Grade 2]

One main spheroid and one small satellite from one well

[Grade 3]

3 or more spheroids or badly deformed ones from one well

[Grade 4]

No spheroid formation

Manufacturer Product Grade 1 Grade 2 Grade 3 Grade 4
Company A Product A 23 3 70 0
Company B Product B 65 27 4 0
Company C Product C 83 8 5 0
Company D Product D 88 6 1 1
Company E Product E 95 1 0 0
S-BIO
Sumitomo Bakelite Co., Ltd.		 PrimeSurface™ 96 0 0 0

Remark

  • Values and images used in this application note is measured values at our company, not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.

※PrimeSurface™ has the advantage of supporting single well single spheroid formation

Radius* of Grade 1 Spheroids

Product A**
(n=23)		 Product B
(n=65)		 Product C
(n=83)		 Product D
(n=88)		 Product E
(n=95)		 PrimeSurface™
(n=96)
Ave (μm) 225.4 244.4 254.8 238.7 261.6 240.4
SD 16.5 11.3 11.7 10.1 9.4 7.3

Remark

  • * Radius was calculated from the Day3 spheroid area, assuming that spheroid was sphere
  • ** Data of Product A was from Day2 spheroids
  • Values and images used in this application note is measured values at our company, not the values for guaranteeing. In addition, these data do not gurantee the product performance under any conditions.

Box plot of Radius* of Grade 1 Spheroids (average=100)

※PrimeSurface™ has the advantage of supporting uniform spheroids formation

【PrimeSurface™ is a great tool to obtain highly reproductive data
by providing uniform spheroids in size】

For Tissue engineering and Regenerative medicine:

Regenova is a novel robotic system that facilitates the fabrication of three- dimensional cellular structures by placing cellular spheroids in fine needle arrays according to pre-designed 3D data. Followings are examples of such fabrications using S-BIO's PrimeSurface™ 96U plate and Bio 3D Printer, Regenova (Cyfuse Biomedical K.K.).; neural 3D tissue and 3D tissue with mesenchymal stem cells.

Neural 3D tissue

Cell sources : hiPSC derived neural progenitor cells
Quantity of cells : 4 X 104 cells/well
Culture Medium : For neural cells
Maturation duration on Well Plate : 2 days
3D structure of printed 3D tissue : printed with 3 X 3 X 2
Quantity of spheroids used for 3D tissue : 18 spheroids
Maturation duration after 3D printing : Remove needles 9 days after 3D print

Spheroid

After 3D print

3D tissue after removal of needles

3D tissue with mesenchymal stem cells

Cell sources : human adipose tissue derived mesenchymal stem cells (hADSC)
Quantity of cells : 5 X 103 cells/well
Culture Medium : For MSC
Maturation duration on Well Plate : 2 days
3D structure of printed 3D tissue : Circle shape with 48 spheroids x 10 layers
Quantity of spheroids used for 3D tissue : 480 spheroids
Maturation duration after 3D printing : Remove needles 6 days after 3D print

Spheroid

After 3D print, from above

After 3D print, side view

3D tissue after removal of needles

Application example of hES cells differentiation using PrimeSurface
The uniform spheroids form spontaneously within one day for effective aggregate formation of ES cells by using PrimeSurface

Self-formation of neural retina tissue from the aggregate of human ES cells by using PrimeSurface MS-9096V

Culture plate : PrimeSurface MS-9096V
Cell type : Human ES cells (KhES-1 strain)
Seeding density : 9,000 cells/well
Culture medium : GMEM+KSR+NEAA+2ME+ 20uM Y-27632
Culture environment : 5%CO2, 37°C

Data resource

  • Picture a-c
    Division of Human Stem Cell Technology RIKEN Center for Developmental Biology

Reference

  • Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs; NakanoT, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y; Cell Stem Cell, 10 (6), 771-785 (2012)

Evaluation example of anticancer drug efficacy by using PrimeSurface

Cell type : MCF-7 (Human breast cancer cell)
Anticancer drug : 5-Fluorouracil (5-FU)

Data resource

Nishio Lab., Department of Genome Biology, Kinki University School of Medicine

Please check the supplemental data for more detail example of application.

References

Introduction of PrimeSurface™ Research Papers

  • Click here to view the PDF file.

Application Examples in Stem Cell Research (PDF 995KB)

Supplemental Data

Time-lapse imaging of human iPS cells spheroid (embryoid body) formation

[Culture conditions]

Culture plate :  PrimeSurface MS-9096V
Cells : hiPS cell (201B7: Takahashi K et al. Cell, 2007 Nov 30;131(5):861-72, iPS Academia Japan, Inc.)
Seeding density : 9,000 cells/well
Culture medium : DMEM/F12 + 20% (v/v) KSR + 1% (v/v) NEAA + L-Glutamine (2mM) + Β-Mercaptethanol (80μM) + Y-27632(30μM)
Culture environment : 5%CO2, 37°C

Time-lapse imaging of HeLa cells spheroid formation in 96 well plate

[Culture conditions]

Seeding density : 1,000 cells/well
Culture medium : MEM+10FBS 100μL/well
Culture period : 48hours

Time-lapse imaging og HepG2 cells spheroid formation in 384 well plate

[Culture conditions]

eding density : 1,000 cells/well
Culture medium : DMEM (Low Glucose) + 10%FBS 50μL/well
Culture period : 48hours

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