Comparison of the effects of oxidative and inflammatory stresses on rat chondrocyte senescence

Introduction PrimeSurface Research Papers

Comparison of the effects of oxidative and inflammatory stresses
on rat chondrocyte senescence

Misaki Yagi , Kentaro Endo, Keiichiro Komori and Ichiro Sekiya. Sci. Rep. 13, 7697 (2023).

https://doi.org/10.1038/s41598-023-34825-1

Copyright: © Yagi, M., Endo, K., Komori, K. and Sekiya, I. 2023
This article is licensed under a Creative Commons Attribution 4.0 International License (CC BY).

Background

Osteoarthritis (OA) is a condition characterized by degeneration and deformity of joint tissues, such as bones and in joint cartilage has been associated with the developmencartilage. It is an age-related disease that can significantly impact quality of life. The loss of homeostasis of OA, and it has been found that chondrocyte senescence is an important factor in the onset and progression of the disease. In this study, effects of oxidative and inflammatory stresses on rat chondrocyte senescence have been investigated.

Research Achievements

From this study, it showed that oxidative and inflammatory stresses increased the SA-β-gal activity in rat chondrocytes. Oxidative stress impaired ECM-producing ability and inhibited the proliferation of rat chondrocytes, while inflammatory stress increased the expression of SASP* factors and the degradation of ECM as well. SASP is the most important senescence phenotype in OA progression because it triggers an imbalance in ECM production and degradation. These findings suggest that inflammatory stress, rather than oxidative stress, is the dominant driver of SASP in OA.

Use of PrimeSurface™ in this study

Rat chondrocyte 3D spheroids were formed using PrimeSurface™ 96U plates. Spheroids were treated with oxidative (H₂O₂) and inflammatory (IL/TNF) stresses and effects on cartilage ECM were evaluated.

Cell density	:	2.5x10⁴ cells/well
Medium	:	chondrogenic medium
Culture period	:	2 weeks
Stress treatment	:	5 days
Analysis method	:	histological staining and Glycosaminoglycan (GAG) & DNA Quantification

[Results]
Fig.5, 6
A: Experimental schema
B: Immunostaining
C: Quantification of GAG and DNA

【Fig.5】

* For details, please refer to the paper

* SASP (senescence-associated secretory phenotype): is a phenotype associated with senescent cells wherein those cells secrete cytokines, growth factors, proteases, etc.