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EZGlyco® O-Glycan Prep Kit論文紹介

Label-Free Liquid Chromatography-Mass Spectrometry Quantitation
of Relative N- and O-Glycan Concentrations in Human Milk in Japan

Toshiyuki Yamaguchi, Hirofumi Fukudome, Junichi Higuchi, Tomoki Takahashi, Yuta Tsujimori, Hiroshi M. Ueno,
Yasuhiro Toba and Fumihiko Sakai, Int. J. Mol. Sci. 25, 1772 (2024)

https://doi.org/10.3390/ijms25031772

Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.

Comprehensive and large-scale study of N/O-glycans in human milk

Background

Human milk is abundant in carbohydrates and includes human milk oligosaccharides (HMOs) and N/O-glycans conjugated to proteins. HMO compositions and concentrations vary in individuals according to the maternal secretor status based on the fucosyltransferase 2 (FUT2) genotype; however, the profile of N/O-glycans remains uninvestigated because of the analytical complexity.

Research Achievements

The authors applied a label-free chromatography-mass spectrometry (LC-MS) technique to elucidate the variation in the composition and concentration of N/O-glycans in human milk. They used label-free LC-MS to relatively quantify 16 N-glycans and 12 O-glycans in 200 samples of Japanese human milk (1-2 months postpartum) and applied high performance anion exchange chromatography with pulsed amperometric detection to absolutely quantify the concentrations of 11 representative HMOs.

Cluster analysis of the quantitative data revealed that O-glycans and several HMOs were classified according to the presence or absence of fucose linked to galactose while N-glycans were classified into a different group from O-glycans and HMOs. O-glycans and HMOs with fucose linked to galactose were more abundant in human milk from secretor mothers than from non-secretor mothers. Thus, secretor status influenced the composition and concentration of HMOs and O-glycans but not those of N-glycans in human milk.

Figure 1. Modification features of N-glycans (A) and O-glycans (B) detected in human milk via label-free LC-MS.

* For details, please refer to the paper

Conclusions

This quantitative analysis of N/O-glycans in human milk demonstrated that secretor status not only influences the composition and concentration of HMOs but also those of O-glycans rather than those of N-glycans in human milk. The relationship between the concentration of N/O-glycans in human milk and infant health and growth is particularly interesting. These questions will be addressed in future studies as information on infant health and development was also collected in this human milk cohort study.

Product Information

Item No. Product name Contents
BS-41601Z EZGlyco® O-Glycan Prep Kit For 10 tests

注記

  • Storage: 4℃
  • Expiration: 2 years after production

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